Description:
Epiphyllum oxypetalum (DC.) or Wijaya Kusuma (Cactaceae) is an ornamental plant which is known to contain polyphenol compounds. The purpose to determine the levels of total phenolic and flavonoid compounds in the fractions of Wijaya Kusuma leaves using microplate-based assay. Each sample was determined phenolic content with Folin-ciocalteu reagent and flavonoid content using AlCl3 on the microplate. Absorption was measured using a microplate reader. Screening of DPPH radical scavenging activity was identified using the TLC method. The results showed that the extract and fractions of the leaves of Wijaya Kusuma contained phenolic and flavonoid compounds. The ether fraction had the highest phenolic content (35,912±0,54776 mgGAE/g) and flavonoids (0,773±0,008 mgQE/g) compared to other fractions (ether>water>ethyl acetate fraction). There is one yellow spot with an Rf value of 0,85 in the ether fraction which is thought to be a compound capable of scavenging the DPPH radicals. The ether fraction of Wijaya Kusuma leaves has potential as a source of polyphenols with antioxidant activity. However, further research is needed to evaluate the antioxidant capacity in the ether fraction of Wijaya Kusuma leaves.

URL:
http://103.158.96.210:88/web_repository/uploads/35828-170559-2-PB.pdf

Type:
Journal

Document:
Diploma III Farmasi

Date:
23-06-2024

Author:
NI P E HIKMAWANTI