Description:
Background: Fibroblast growth factors (FGFs) are involved in angiogenesis, wound healing and embryonic development. However, one of the causes of cancer cell growth in fibroblastdependent cancers is FGF7 secreted by fibroblasts. Therefore, antibodies against FGF7 can be used for the treatment of these types of cancers. Methods: In the previous studies, a phage displaying single domain antibody, D53, against human FGF7 has been identified using the phage display technique. In the present study, D53 was produced and purified in its isolated form. ELISA experiment was performed to evaluate the binding of D53 to FGF7. The mode of interaction of D53-FGF7 was explored using docking study and molecular dynamics (MD) simulations. Results: The expression and purification processes were verified using western blotting and SDS-PAGE analyses. ELISA experiment showed that D53 is able to recognize and bind FGF7. Docking study and MD simulations indicated that compared to dummy VH, D53 has more affinity towards FGF7. Conclusion: The findings in the current study can be useful for the generation and the development of FGF7 inhibitors with a potential use in fibroblast-dependent cancers.

URL:
http://103.158.96.210:88/web_repository/uploads/ps-28-405.pdf

Type:
Journal

Document:
Diploma III Farmasi

Date:
23-06-2024

Author:
Ali Akbar Alizadeh