Description:
Objective: The popularity of Sildenafil, the widespread distribution of various products and dietary supplements with added synthetic drugs, requires reliable analysis methods. This research study aimed to develop a simple isocratic HPLC method for the determination of Sildenafil in tablet dosage forms from the local market. Methods: Separation was carried out at 30 °C, using column LiChrosorb® RP-18 (150 x 4.0 mm, 5 ?m) with mobile phase consisting of acetonitrile: methanol: 0.5% triethylamine (15: 26: 59 v/v/v). The detector was set at 290 nm. The flow rate was 1.0 ml/min and the injection volume was 20 ?l. Results: Linear correlation was obtained within the range 6.25–50.0 ?g/ml with correlation coefficient (R2) 0.9998. The achieved limits of detection and quantitation were 0.7 and 2.2 ?g/ml, respectively. Conclusion: The developed method can be applied for the quality control of Sildenafil preparations.

URL:
http://103.158.96.210:88/web_repository/uploads/editorijap,+39719-Article+Text-186803-1-6-20201016.pdf

Type:
Journal

Document:
Diploma III Farmasi

Date:
23-06-2024

Author:
MIGLENA SMERIKAROVA