Description:
Context: Diabetes is a chronic health condition with insulin disturbance. Hibiscus surattensis L. is a medicinal plant and plays a pivotal role in diabetes. Aims: To evaluate the effect of H. surattensis leaves extract to increase insulin secretion and repair pancreatic tissue damage in streptozotocin (STZ)-induced diabetic mice. Methods: This research used male mice (Swiss Webster) administrated by STZ 100 mg/kg BW to induce diabetes. Animals were divided into eight groups (n = 6) consisting of normal control, diabetic control, glibenclamide, ethanol extract 50, ethyl acetate fraction, and water fraction with doses of 25 and 50 mg/kg BW, respectively. Treatment was given for 28 days. The evaluation was carried out on blood glucose levels, insulin levels, HOMA-?, and the pancreatic islets by the morphometric analysis were evaluated. All data were analyzed using SPSS 16.0. Results: Extract and active fraction of H. surattensis leaves, especially ethyl acetate fractions (25 and 50 mg/kg BW) significantly (p<0.05) reduced fasting blood glucose levels, insulin levels, and the strength of pancreatic ? cells (HOMA-?) increased in STZ induced diabetic mice. Histopathological results in pancreatic confirmed the effect of H. surattensis to increase the number of islets significantly in Langerhans and pancreatic ?-cells compared to the diabetic control group (p<0.05). Conclusions: Extract, and active fraction of H. surattensis leaves affect the insulin secretion and repairing the pancreatic tissue damage on diabetic mice, the most optimal therapy on the administration of ethyl acetate fraction.

URL:
http://103.158.96.210:88/web_repository/uploads/jppres20.978_9.4.454.pdf

Type:
Journal

Document:
Diploma III Farmasi

Date:
23-06-2024

Author:
Yuliet