Description:
Context: The pyrolysis product of rice hulls (Oryza sativa) has been used
for the natural preservation of food and shows the potential to act as an
antioxidant and anti-inflammatory product. However, the safety of this
treatment has still not been studied.
Aims: To analyse liquid smoke derived from rice hulls (Oryza sativa)
components, its physical characteristics including acidity, density, and
its toxicity on baby hamster kidney (BHK) cells.
Methods: The physical characteristics of acidity and density were
analysed using a digital pH meter and gravimetry. The components of
liquid smoke from rice hulls were analysed using gas chromatograph
mass spectrometry. Eight concentrations (1, 2.5, 5, 7.5, 10, 12.5, 15 and
17.5%) of liquid smoke from rice hulls were used and immersed in
media culture for 24 hours. Then, the MTT assay method was used to
investigate the cytotoxic effect on BHK cells. Optic formazan density
was indicated from the number of living cells.
Results: The acidity of liquid smoke from rice hulls was 2.296 and the
density is 1.0102 g/mL. The components analysed were 2-
methoxyphenol (13.45%), mequinol (13.45%) and phenol (10.52%). The
10% concentration showed the highest number of living cells at 49.33%,
while liquid smoke rice hulls had 12.5% of cells still alive (p=0.000),
followed by the concentration of 7.5% (p=0.000), concentration of 5%
(p=0.000) and liquid smoke rice hulls at 1% (p=0.000).
Conclusions: Liquid smoke from rice hulls (Oryza sativa) showed that it’s
potential of toxicity may be related its high acidity, components and the
concentration.
URL:
http://103.158.96.210:88/web_repository/uploads/jppres20.928_9.1.78.pdf
Type:
Journal
Document:
Diploma III Farmasi
Date:
23-06-2024
Author:
Ira Arundina