Description:
An analytical method for the determination of thiamphenicol in dry syrup has been developed by the TLC video densitometry method. Identification and quantification of thiamphenicol in simple pharmaceutical preparations were performed on a silica gel GF254 plate using mobile phase ethyl acetate:chloroform:methanol (7.5:2:0.5, v/v/v). The detection was carried out under a UV lamp at 254 nm and then captured by a digital camera with ISO 100 sensitivity, 50 mm focal point, f/6 aperture, and 1/15 sec shutter speed. The captured images were analyzed using ImageJ software to determine the AUC values of each spot. The selectivity result was 1.52. Method validation results obtained regression equation y = 1.7481x + 16498 (r = 0.9995; Vx0 = 0.80%). The limit of detection and the limit of quantitation were 21.78 and 72.60 ppm respectively. The precision (%RSD) intra-day and inter-day precisions were 1.07 and 1.34% respectively. The method has mean recoveries in the range of 96.62 - 100.91%. The levels of thiamphenicol in three dry syrup samples were 99.26, 101.25, and 101.81%, respectively. The TLC video densitometry method can be used as a routine analysis of thiamphenicol in dry syrup.

URL:
http://103.158.96.210:88/web_repository/uploads/50619-201405-1-PB.pdf

Type:
Journal

Document:
Diploma III Farmasi

Date:
23-06-2024

Author:
WINASIH RACHMAWATI