Description:
Many plants are used ethnomedicinally for the treatment of disease and restoration of health. One of these medicinal plants is Tithonia diversifolia. This study aims to determine the phenolic and flavonoid contents and antioxidant activity using the DPPH method from the leaves of T. diversifolia. Extraction was carried out by the maceration method using 70% ethanol and ethyl acetate as solvents. Phenolic identification was carried out with FeCl3 reagent; the blackish green color formed indicates the presence of phenolic compounds. Identification of flavonoid was carried out using a reagent between magnesium powder and concentrated HCl; the orange-red color formed indicated the presence of flavonoid. Determination of phenolic and flavonoid contents from extract of T. diversifolia leaves was carried out colorimetrically (AlCl3 and NaCH3) using a UV-Vis spectrophotometer. The results showed that the total phenolic contents of the ethyl acetate and ethanolic extract of the T. diversifolia leaves was 1.28 and 1.37% GAE, respectively, while the total flavonoid contents was 2.21 and 3.41 mgQE/g, respectively. Better antioxidant activity was shown by the ethanol extract with an IC50 value of 84.018 µg/ml. This information can be used to develop T. diversifolia leaves extract as an antioxidant agent because it has intense inhibition against free radicals by the DPPH method

URL:
http://103.158.96.210:88/web_repository/uploads/36900-133944-1-PB.pdf

Type:
Journal

Document:
Diploma III Farmasi

Date:
23-06-2024

Author:
NOER F RAHMAN