Repository Akfar Bumi Siliwangi



Determination of Gamma Oryzanol from Ethanolic Extract of Indonesian Rice Bran (Oryza sativa)



Description:
Rice bran contains gamma oryzanol, a chemical compound mostly consisted of complex ester transferulate with phytosterols. Gamma oryzanol has a strong antioxidant activity, explained by the free radical scavenging activity. This study was aimed to determine gamma oryzanol from ethanolic extract of Indonesian rice bran. Quantitative analysis was conducted using HPLC analysis with mixture of met hanol:acetonitrile:isopropanol (50%:40%:10%) as mobile phase and Inertsil ODS-3 5 µm as stationary phase. The UV detector wavelength was set to 327 nm, and the flow rate was set to 1 mL/min. Gamma oryzanol standard and rice bran ethanolic extract 96% was injected into HPLC system. Ethanolic extract of rice bran (Oryza sativa) sample showed four major peaks as those of gamma oryzanol standard and contained 105,981 ppm of gamma oryzanol. As the conclusion, ethanolic extract of Indonesian rice bran (Oryza sativa) contains 10.59% gamma oryzanol

URL:
http://103.158.96.210:88/web_repository/uploads/46297-203561-2-PB.pdf

Type:
Journal

Document:
Diploma III Farmasi

Date:
23-06-2024

Author:
AFIFAH K VARDHANI