Description:
Rice bran contains gamma oryzanol, a chemical compound mostly consisted of complex ester transferulate with phytosterols. Gamma oryzanol has a strong antioxidant activity, explained by the free
radical scavenging activity. This study was aimed to determine gamma oryzanol from ethanolic extract
of Indonesian rice bran. Quantitative analysis was conducted using HPLC analysis with mixture of met
hanol:acetonitrile:isopropanol (50%:40%:10%) as mobile phase and Inertsil ODS-3 5 µm as stationary
phase. The UV detector wavelength was set to 327 nm, and the flow rate was set to 1 mL/min. Gamma
oryzanol standard and rice bran ethanolic extract 96% was injected into HPLC system. Ethanolic extract
of rice bran (Oryza sativa) sample showed four major peaks as those of gamma oryzanol standard and
contained 105,981 ppm of gamma oryzanol. As the conclusion, ethanolic extract of Indonesian rice
bran (Oryza sativa) contains 10.59% gamma oryzanol
URL:
http://103.158.96.210:88/web_repository/uploads/46297-203561-2-PB.pdf
Type:
Journal
Document:
Diploma III Farmasi
Date:
23-06-2024
Author:
AFIFAH K VARDHANI