Description:
Melasma is a pigmentation disorder caused by excess melanin synthesis in the form of brown patches around the face or neck. Melasma can affect all racial groups, especially areas with high ultraviolet radiation. Substances that are often used to prevent hyperpigmentation are kojic acid, azelic acid and mercury, but these substances are carcinogenic if used continuously. Red betel (Piper crocatum) has active compounds that act as antioxidants such as flavonoids and phenols. This study aims to determine the potential of the active compound of red betel leaves against tyrosinase inhibition. This research was conducted using in silico and in vitro methods. The receptors used were tyrosinase with PDB code 5M8O, tropolone as native ligand, and kojic acid as comparison ligand. A total of 34 active compounds of red betel leaf from LC-MS and GC-MS analysis were used as test ligands. Molecular docking was performed using AutoDock Vina and AutoDock Tools, and visualized using Ligplot+ and PyMOL. In vitro tyrosinase inhibition used the tyrosinase activity assay kit ab252899 and kojic acid as a positive control. The catechin compound has the smallest binding free energy (?G), which is ?6.7 kcal/mol, and the kinetics of inhibition is 12.12 M. In vitro test results, the water fraction has the largest tyrosinase inhibitory activity of 84.84% and kojic acid has an activity of 82.47%.

URL:
http://103.158.96.210:88/web_repository/uploads/44673-203496-2-PB.pdf

Type:
Journal

Document:
Diploma III Farmasi

Date:
23-06-2024

Author:
SYAHRUL MUSTOPA