Description:
Cordia sebestena L. contains flavonoids, that play a role in free radical scavenging activity. This study aimed to analyze the flavonoids in the hydrolyzed fraction of the 70% ethanol extract of Cordia leaves. The study was divided into 7 stages: (1) maceration using 70% ethanol, (2) acid hydrolysis of the ethanol extract, (3) fractionation of hydrolysate with ethyl acetate, (4) determination of total flavonoid content, (5) antiradical activity assay against 2,2?-diphenyl-1-picrylhydrazyl (DPPH), (6) thin layer chromatography (TLC) analysis, and (7) identification of flavonoid using UV-Visible Spectrophotometry and Infrared Spectroscopy. The total flavonoid content of the ethyl acetate fraction was 8.20 mgQE/g with an IC50 value of 296.91 g/mL against DPPH radicals. There is one spot of flavonoids in the ethyl acetate fraction (hRf = 85.5). The UV-Vis spectrum on the spot obtained absorption at 340 nm (band I) and 240 nm (band II). The infrared spectrum shows a signal at 3363 cm-1 (O-H stretching), 1727 cm-1 (C=O), and 1060 cm-1 strong (C-O). The ethyl acetate hydrolysate fraction of Cordia leaf contains flavonoid similar to the chalcone group. Further analysis is needed using 1 H NMR or FTIR data from a known chalcone as a standard to confirm the flavonoid

URL:
http://103.158.96.210:88/web_repository/uploads/38849-203464-2-PB.pdf

Type:
Journal

Document:
Diploma III Farmasi

Date:
23-06-2024

Author:
NI P E HIKMAWANTI